monoclonal polyclonal anti ha antibody  (Covance)

Journal: Science Advances

Article Title: Repeated oral exposure to H5N1 influenza virus in pasteurized milk does not cause adverse responses to subsequent influenza infection

doi: 10.1126/sciadv.aeb3906

Figure Lengend Snippet: ( A ) Pasteurization of virus at either high temperature (72°C for 15 s) or low temperature (65°C for 30 min). H1N1 CA/09 or recombinant HA protein as a positive control was ran on a 4 to 20% gel and stained for total protein using Coomassie blue. ( B ) Samples were diluted (1:50) of pasteurized milk in PBS, live H1N1 virus in PBS, or H1N1 virus pasteurized in either PBS or milk for 72°C for 15 s and were incubated with a combination of a monoclonal antibody (mAb) and polyclonal antibody (pAb) against the H1N1 HA. ( C ) Analysis of protein expression relative to milk in PBS control. ( D ) HA amino acid sequence comparison between CA/09 H1N1 and A/bovine/Ohio/2024 (H5N1) with identical sequences highlighted in blue and additional similar residues highlighted in yellow. ( E ) In silico analyses of the H1N1 or H5N1 HA amino acid sequences that are important for protein stabilization and melting temperature. Statistical analysis includes (C) one-way ANOVA with Tukey’s multiple comparisons. * P < 0.05; **** P < 0.0001; n.s., not significant. Data are shown as mean with SD.

Article Snippet: They were then probed for protein with a combination of anti-HA monoclonal and polyclonal antibodies (Sino Biological, 11055-RM10 and 11055-T62).

Techniques: Virus, Recombinant, Positive Control, Staining, Incubation, Expressing, Control, Sequencing, Comparison, In Silico